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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
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Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...

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Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...

Ribosomes

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Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...

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The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...

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Decoding and recoding the ribosomal peptide universe.

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|December 25, 2012

Summary

This summary is machine-generated.

Researchers have engineered new thiopeptide antibiotics using ribosomal peptide natural products (RiPPs). This breakthrough overcomes previous limitations, enabling in vivo synthesis of these valuable bioactive small molecules.

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Area of Science:

Biochemistry and Molecular Biology
Natural Product Drug Discovery

Background:

Ribosomal peptide natural products (RiPPs) represent a diverse class of bioactive small molecules.
The discovery and engineering of RiPPs have been historically challenging due to various biological and chemical hurdles.

Discussion:

Young and colleagues have successfully addressed key limitations in RiPP development.
Their work facilitates the in vivo production of complex thiopeptide antibiotics.

Key Insights:

Overcoming developmental hurdles in RiPP synthesis.
Enabling in vivo production of novel thiopeptide antibiotics.
Expanding the toolkit for natural product drug discovery.

Outlook:

Potential for discovering and engineering a wider range of RiPP-based therapeutics.
Accelerated development of new antimicrobial agents.
Advancements in synthetic biology for bioactive molecule production.