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Updated: Aug 24, 2026

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Electroeluting DNA Fragments
Published on: September 5, 2010
Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure
Nucleic Acids Research
|January 25, 1990
Abstract:
Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate. The DNA obtained following the present protocol transfects eukaryotic cells with higher efficiency.

