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Updated: May 15, 2026

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Published on: February 25, 2021
Microscale separation of heparosan, heparan sulfate, and heparin
Xue Zhao1, Bo Yang, Kathryn Linkens
1College of Food Science and Technology, Ocean University of China, Qingdao 266003, People's Republic of China.
Abstract:
The separation and quantification of glycosaminoglycan (GAG) chains with different levels of sulfation from cells and media, and prepared through chemoenzymatic synthesis or metabolic engineering, pose a major challenge in glycomics analysis. A method for microscale separation and quantification of heparin, heparan sulfate, and heparosan from cells is reported. This separation relies on a mini strong anion exchange spin column eluted stepwise with various concentrations of sodium chloride. Disaccharide analysis by LC-MS was used to monitor the chemical structure of the various GAG chains that were recovered.
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