Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Constructing deletions with defined endpoints in Drosophila.

L Cooley1, D Thompson, A C Spradling

  • 1Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210.

Proceedings of the National Academy of Sciences of the United States of America
|April 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stem cells and their niches: integrated units that maintain Drosophila tissues.

Cold Spring Harbor symposia on quantitative biology·2008
Same author

The receptor-like tyrosine phosphatase lar is required for epithelial planar polarity and for axis determination within drosophila ovarian follicles.

Development (Cambridge, England)·2001
Same author

The nuclear location and chromatin organization of active chorion amplification origins.

Chromosoma·2001
Same author

Mouse ovarian germ cell cysts undergo programmed breakdown to form primordial follicles.

Developmental biology·2001
Same author

Exercise-based rehabilitation for coronary heart disease.

The Cochrane database of systematic reviews·2001
Same author

Item-cued directed forgetting of related words and pictures in children and adults: selective rehearsal versus cognitive inhibition.

The Journal of general psychology·2001

Researchers developed a new method for creating precise chromosomal deletions in Drosophila. This technique uses P transposable elements to efficiently generate deletions, advancing Drosophila genetics research.

Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Chromosomal deficiencies are essential tools in Drosophila genetics for studying gene function and mapping mutations.
  • Previous methods for constructing deficiencies were often inefficient or lacked precision.

Purpose of the Study:

  • To develop and validate a novel, efficient method for constructing precise chromosomal deficiencies in Drosophila.
  • To leverage P transposable elements for targeted deletion generation.

Main Methods:

  • Two P transposable elements were strategically placed at desired deletion endpoints in progenitor Drosophila strains.
  • P element transposase was expressed to induce deletions between the flanking P elements.
  • The configuration of P elements (cis vs. trans) was analyzed for its effect on deletion recovery.

Related Experiment Videos

Main Results:

  • High-frequency recovery of deletions spanning the P elements was achieved when they were in a cis configuration.
  • The P element transposase-mediated method demonstrated efficiency in generating precise chromosomal deficiencies.
  • This approach allows for the targeted deletion of virtually any chromosomal region.

Conclusions:

  • The developed method provides an efficient and precise way to construct Drosophila chromosomal deficiencies.
  • The extensive collection of P element insertion lines facilitates the targeted deletion of any desired genomic region.
  • This technique significantly advances the capabilities for genetic analysis in Drosophila.