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Updated: May 15, 2026

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
Published on: June 14, 2013
Single-molecule analysis of gene expression using two-color RNA labeling in live yeast
Sami Hocine1, Pascal Raymond, Daniel Zenklusen
1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA.
Abstract:
Live-cell imaging of mRNA yields important insights into gene expression, but it has generally been limited to the labeling of one RNA species and has never been used to count single mRNAs over time in yeast. We demonstrate a two-color imaging system with single-molecule resolution using MS2 and PP7 RNA labeling. We use this methodology to measure intrinsic noise in mRNA levels and RNA polymerase II kinetics at a single gene.

