A versatile genome-scale PCR-based pipeline for high-definition DNA FISH
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Updated: May 15, 2026

High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
Published on: June 29, 2018
Magda Bienko1, Nicola Crosetto1, Leonid Teytelman1
1Departments of Physics and Biology, and the Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Abstract:
We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.

