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Effects of MnTnHex-2-PyP on lung antioxidant defence system in asthma mice model
Violeta Dancheva1, Lyudmil Terziev, Veneta Shopova
1Sector of Disaster Medicine, Medical University, Pleven, Bulgaria. vilidana@abv.bg
Abstract:
We aimed to study the MnTnHex-2-PyP effect on some markers of lung antioxidant defence system in mice asthma model.The study was carried out on 28 C57B1/6 mice divided into four treatment groups: group 1 - controls; group 2 - injected and inhaled with ovalbumin; group 3 - treated with MnTnHex-2-PyP and inhaled with phosphate buffered saline; group 4 - injected with ovalbumin and MnTnHex-2-PyP but also inhaled with ovalbumin. On days 24, 25 and 26, mice from groups 1 and 2 were inhaled with PBS for 30 min, and those from groups 2 and 4 were given a 1% ovalbumin solution. One hour before inhalation, and 12 hours later the animals from groups 1 and 2 were injected i.p. with 100 μl PBS, and those from groups 3 and 4 received a 100 μl MnTnHex-2-PyP solution in PBS, сontaining 0,05mg/kg. The animals were killed by exsanguination 48 hours after the last inhalation for obtaining a lung homogenate. The activities of superoxide dismutase, catalase, glutathione peroxidase and the non-protein sulphhydryl group content in the lung homogenate were investigated. Ovalbumin decreased the activities of superoxide dismutase (p=0.01), catalase (p=0.002), glutathione peroxidase and non-protein sulphhydryl groups content (p<0.001) in comparison to controls. In group 4 (ovalbumin and MnTnHex-2-PyP) the activities of superoxide dismutase (p=0.044), catalase (p=0.045), glutathione peroxidase (p=0.002), and the non-protein sulphhydryl groups content (p<0.001) were significantly increased compared to ovalbumin (group 2).MnTnHex-2-PyP restored the activities of basic enzymes in the lung antioxidant defence system in ovalbumin-induced asthma mice model, 48 hours after the last nebulization.
Insights
This study shows MnTnHex-2-PyP effectively restores lung antioxidant defense markers in an ovalbumin-induced asthma mouse model. The compound significantly increased key enzyme activities, mitigating asthma-related oxidative stress.
Area of Science:
- Biochemistry
- Pharmacology
- Immunology
Background:
- Asthma is a chronic respiratory disease characterized by airway inflammation and oxidative stress.
- The lung antioxidant defense system plays a crucial role in mitigating oxidative damage.
- Ovalbumin-induced asthma in mice is a widely used model to study asthma pathogenesis and test therapeutic interventions.
Purpose of the Study:
- To investigate the effect of MnTnHex-2-PyP on the lung antioxidant defense system in a mouse model of asthma.
- To evaluate the potential of MnTnHex-2-PyP as a therapeutic agent for asthma by examining its impact on key antioxidant enzymes.
Main Methods:
- A mouse model of asthma was established using ovalbumin sensitization and challenge.
- Mice were divided into four groups: control, ovalbumin-induced asthma, MnTnHex-2-PyP treatment, and ovalbumin-induced asthma with MnTnHex-2-PyP treatment.
- Lung homogenates were analyzed for the activities of superoxide dismutase, catalase, glutathione peroxidase, and non-protein sulfhydryl group content.
Main Results:
- Ovalbumin challenge significantly decreased the activities of superoxide dismutase, catalase, glutathione peroxidase, and non-protein sulfhydryl groups compared to controls.
- Treatment with MnTnHex-2-PyP in ovalbumin-induced asthmatic mice significantly increased the activities of superoxide dismutase, catalase, glutathione peroxidase, and non-protein sulfhydryl groups compared to the ovalbumin-only group.
- These restorative effects were observed 48 hours after the last nebulization.
Conclusions:
- MnTnHex-2-PyP demonstrates a significant protective effect on the lung antioxidant defense system in an ovalbumin-induced asthma model.
- The compound effectively restored the activities of key antioxidant enzymes, suggesting its therapeutic potential for managing oxidative stress in asthma.
