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A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles
Published on: July 11, 2025
Nocardioides marinquilinus sp. nov., isolated from coastal seawater
Yirang Cho1, Gwang Il Jang1, Byung Cheol Cho1
1Microbial Oceanography Laboratory, School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
Abstract:
A Gram-stain-positive, non-motile, strictly aerobic, non-spore-forming and short rod-shaped bacterial strain, CL-GY44(T), was isolated from coastal seawater, Korea. Analysis of the 16S rRNA gene sequence of strain CL-GY44(T) revealed a clear affiliation with the genus Nocardioides. Based on phylogenetic analysis, strain CL-GY44(T) showed the closest phylogenetic relationship with Nocardioides ginsengagri BX5-10(T) and Nocardioides plantarum NCIMB 12834(T). Strain CL-GY44(T) was not able to grow in the presence of NaCl but grew with 0-5.5 % sea salts. The optimum temperature and pH for growth were 30 °C and pH 7.0. The major cellular fatty acids of strain CL-GY44(T) were C17 : 1ω6c, iso-C16 : 0, C16 : 0 and iso-C15 : 0 and the major menaquinone was MK-8(H4). The cell-wall analysis showed that strain CL-GY44(T) contained ll-diaminopimelic acid. The genomic DNA G+C content was 71.6 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain CL-GY44(T) could be clearly distinguished from members of the genus Nocardioides. Thus, strain CL-GY44(T) should be classified as representing a novel species in the genus Nocardioides, for which the name Nocardioides marinquilinus sp. nov. is proposed. The type strain is CL-GY44(T) ( = KCCM 90109(T) = JCM 18459(T)).
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