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Updated: May 15, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
The sequence variation responsible for the functional difference between the CONSTANS protein, and the CONSTANS-like
Soon-Kap Kim1, Hyo-Young Park, Yun Hee Jang
1Plant Signaling Network Research Center, Division of Life Sciences, Korea University, Seoul, Republic of Korea.
Abstract:
Although the protein CONSTANS (CO) and its close relatives CONSTANS-like (COL) 1 and COL2 exhibit high amino acid sequence similarities, only the CO protein regulates floral induction in Arabidopsis. To investigate the structural basis for the functional differences between CO, COL1, and COL2 in flowering, we performed domain-swapping between CO, COL1, and COL2, and site-directed mutagenesis on the first exon of CO. The results suggest that the lack of flowering promotion activity by COL1 and COL2 is mainly attributed to the differences between CO and the COL1 and COL2 proteins in the amino acid sequence encoded by their first exons.
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