Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AI-Integrated Multi-Target Validation of <i>Coreopsis tinctoria</i> Polyphenols as a Functional Food Ingredient Against Diabetic Nephropathy.

Foods (Basel, Switzerland)·2026
Same author

Dicaffeoylquinic acid alleviates alcoholic liver disease by targeting PLA2G4B and inhibiting the MAPK signaling pathway.

Frontiers in pharmacology·2026
Same author

The nutritional evaluation and hypoglycemic activity of chickpea (Cicer arietinum L.) proteins in vivo based on network pharmacology.

Food research international (Ottawa, Ont.)·2026
Same author

Discovery of twelve undescribed diterpenoid alkaloids and identification of shawurensine A as a potent α7 nAChR antagonist.

Phytochemistry·2026
Same author

<i>Artemisia scoparia</i> extract inhibits oxidative stress and ferroptosis to ameliorate MASH through AGE-RAGE and JAK1-STAT3 signaling.

Frontiers in pharmacology·2026
Same author

Design, synthesis, and evaluation of novel tricyclononene carboxamide derivatives as dual anti-orthopoxvirus and anti-inflammatory agents.

Bioorganic & medicinal chemistry·2026

Related Experiment Video

Updated: May 15, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

A sample pretreatment strategy for pH-zone-refining counter-current chromatography.

Yi Yang1, Dongyu Gu, Qibin Chen

  • 1Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.

Journal of Chromatography. A
|December 26, 2012
PubMed
Summary

A novel acid-base extraction pretreatment method significantly enhances compound yields in pH-zone-refining counter-current chromatography (CCC). This technique, demonstrated with Sophora alopecuroides alkaloids, effectively doubles target compound recovery.

More Related Videos

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
11:25

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
05:47

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Published on: June 25, 2020

Related Experiment Videos

Last Updated: May 15, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
11:25

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
05:47

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Published on: June 25, 2020

Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Natural Product Chemistry

Background:

  • Counter-current chromatography (CCC) is a liquid-liquid separation technique.
  • pH-zone-refining CCC requires careful selection of solvent systems for optimal performance.
  • Pretreatment methods can impact the efficiency and yield of CCC separations.

Purpose of the Study:

  • To establish a sample pretreatment method for pH-zone-refining CCC.
  • To increase the yield of target compounds using acid-base extraction.
  • To demonstrate the method's efficacy using alkaloids from Sophora alopecuroides.

Main Methods:

  • Developed an acid-base extraction pretreatment strategy.
  • Utilized a two-phase solvent system (MtBE-CH₃CN-H₂O) with pH adjusted to 10.
  • Applied pH-zone-refining CCC to a pretreated crude alkaloid extract.

Main Results:

  • The pretreatment method substantially increased target compound yields.
  • Yields of sophoramin and sophoearpin were doubled compared to the untreated sample.
  • Isolated 169 mg of sophoramin and 696 mg of sophoearpin from a 10 g extract.

Conclusions:

  • The acid-base extraction pretreatment is effective for enhancing yields in pH-zone-refining CCC.
  • This method offers a significant improvement for isolating target compounds.
  • The developed pretreatment strategy is advantageous for natural product analysis.