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Related Experiment Video

Updated: May 15, 2026

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Published on: January 20, 2017

TAL effectors: function, structure, engineering and applications.

Amanda Nga-Sze Mak¹, Philip Bradley, Adam J Bogdanove

¹Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N. A3-025, Seattle, WA 98109, United States.

Current Opinion in Structural Biology

|December 26, 2012

Summary

This summary is machine-generated.

Transcription activator-like (TAL) effectors are bacterial proteins that bind DNA. Their modular structure allows precise gene targeting, enabling applications like site-specific nucleases.

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Last Updated: May 15, 2026

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

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Published on: January 20, 2017

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Area of Science:

Molecular Biology
Plant Pathology
Genetics

Background:

TAL effectors are proteins secreted by bacterial pathogens into plant cells.
They translocate to the nucleus and regulate plant gene expression.

Purpose of the Study:

To elucidate the DNA recognition mechanism of TAL effectors.
To highlight their potential as gene-targeting reagents.

Main Methods:

Analysis of TAL effector modular architecture.
Crystallographic and biochemical studies of TAL effector-DNA interactions.

Main Results:

TAL effectors possess a modular DNA-binding domain composed of repeats.
Specific residues within these repeats (RVDs) determine DNA base recognition.
This mechanism allows sequential base recognition along DNA strands.

Conclusions:

The modular structure of TAL effectors is key to their DNA-binding specificity.
Understanding this mechanism facilitates their use as engineered gene-targeting tools, including nucleases.