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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Exploring charged biased regions in the human proteome.
1Molecular and Cellular Diagnosis Processes, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, Po Box 1177, 3018 Sfax, Tunisia.
Gene
|December 26, 2012
Summary
Charged regions in human proteins are linked to crucial roles, including DNA binding and regulation. This study identified 446 charged biased proteins, highlighting their functional significance in the human proteome.
Area of Science:
- Proteomics
- Bioinformatics
- Genomics
Background:
- Biased regions in proteins are increasingly recognized for their structural and functional importance.
- These regions are often associated with protein disorder or specific cellular roles.
Purpose of the Study:
- To investigate charged biased protein sequences within the human genome.
- To identify and characterize the functions associated with these charged regions.
Main Methods:
- Analysis of the human proteome to identify charged biased protein sequences.
- Utilized Gene Ontology analysis to determine the functions of identified proteins.
Main Results:
- Identified 446 charged biased proteins in the human proteome.
- Negative charge runs are prevalent in transcription factors, importins, and protein kinases.
- Positive charge clusters are significantly associated with ribosomal proteins.
- Proteins with zinc-binding fingers often exhibit mixed charged regions.
- Gene Ontology analysis indicates charged proteins are primarily involved in regulatory functions.
Conclusions:
- Charged biased regions are critical determinants of protein function in humans.
- These regions play key roles in DNA binding, protein transport, and cellular regulation.
- The distribution and characteristics of charged regions provide insights into protein evolution and function.
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