A prototype 'Infucon' device for continuous infusion of microbubbles in vivo

Area of Science:

• Medical Devices
• Ultrasound Contrast Agents
• Preclinical Research

Background:

• Microbubbles (MBs) are crucial for ultrasound imaging, but their administration can be challenging.
• Flotation and accumulation of MBs in syringes can lead to inhomogeneous delivery.
• Optimizing MB delivery is essential for improving diagnostic ultrasound efficacy.

Purpose of the Study:

• To design, construct, and evaluate a novel device, 'Infucon', for continuous microbubble infusion.
• To assess the safety and efficacy of the Infucon device in a preclinical rabbit model.
• To compare the duration of ultrasound signal enhancement using continuous infusion versus bolus injection of microbubbles.

Main Methods:

• The Infucon device was developed to prevent microbubble flotation and ensure homogeneous intravenous (i.v.) application.
• Sixteen male New Zealand White rabbits were used for testing, with an average weight of 3.5 kg.
• Two types of microbubbles were administered: commercial SonoVue and laboratory-prepared pegylated DPPC microbubbles, using sulfur hexafluoride as the filling gas.

Main Results:

• Continuous infusion of microbubbles using the Infucon device prolonged the ultrasound signal in the rabbit heart to 12 minutes, significantly longer than the approximately 1 minute observed with bolus application.
• No adverse effects were observed in the rabbits following microbubble administration via the Infucon device.
• The Infucon device demonstrated effective and homogeneous delivery of microbubbles.

Conclusions:

• The Infucon device provides a safe and effective method for continuous microbubble infusion.
• This technology has the potential to significantly improve ultrasound imaging by extending the duration of contrast enhancement.
• The principles of the Infucon device could form the basis for future clinical applications in diagnostic ultrasound.