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Updated: May 15, 2026

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
09:07

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Published on: December 19, 2018

LATS2 is a modulator of estrogen receptor alpha

Lei Cheng Lit1, Susan Scott, Hua Zhang

  • 1Department of Surgery & Cancer, Imperial College, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK.

Anticancer Research
|December 26, 2012
PubMed
Abstract

Insights

Large tumor suppressor homolog-2 (LATS2) kinase modulates Estrogen Receptor α (ERα) activity, impacting ERα-regulated gene transcription and breast cancer cell proliferation. This finding is relevant for developing new endocrine therapies.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Endocrinology

Background:

  • Estrogen Receptor α (ERα) is crucial in breast cancer, with resistance to endocrine therapy a significant clinical challenge.
  • ERα phosphorylation is implicated in endocrine resistance mechanisms.
  • Large tumor suppressor homolog-2 (LATS2) was identified as a potential kinase targeting ERα.

Purpose of the Study:

  • To investigate the role of LATS2 in ERα transcriptional activation.
  • To determine the functional consequences of LATS2 modulation on ERα activity and breast cancer cell behavior.

Main Methods:

  • Utilized molecular and cellular biology techniques.
  • Examined LATS2's effect on ERα-regulated gene expression and cell proliferation.
  • Analyzed protein level changes including cyclin-D1, NCoR, and p27.

Main Results:

  • LATS2 co-localizes with ERα in the nucleus.
  • LATS2 silencing enhanced ERα-regulated gene expression and inhibited cell proliferation.
  • LATS2 inhibition decreased cyclin-D1 and NCoR, while increasing p27 expression.

Conclusions:

  • LATS2 modulates ERα-regulated gene transcription via direct and/or indirect interactions.
  • Understanding LATS2's role in ERα signaling offers potential therapeutic avenues for breast cancer.

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