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DNA methylation of RUNX3 in papillary thyroid cancer

Hee Ja Ko1, Bo Yeon Kim, Chan Hee Jung

  • 1Department of Internal Medicine, Soonchunhyang University College of Medicine, Cheonan, Korea.

The Korean Journal of Internal Medicine
|December 28, 2012
PubMed
Abstract

Insights

Runt-related transcription factor 3 (RUNX3) gene hypermethylation is linked to thyroid cancer. RUNX3 gene silencing may occur in thyroid tumors, and its methylation could serve as a diagnostic marker for papillary thyroid cancer.

Area of Science:

  • Molecular oncology
  • Cancer epigenetics
  • Thyroid cancer research

Background:

  • Runt-related transcription factor 3 (RUNX3) gene inactivation is implicated in various solid tumors.
  • Limited data exists on the role of RUNX3 in the development of thyroid cancers.

Purpose of the Study:

  • To investigate the DNA methylation status of the RUNX3 gene in thyroid cancer.
  • To explore the correlation between RUNX3 gene expression and its methylation in thyroid cancer cell lines.

Main Methods:

  • Evaluated RUNX3 DNA methylation in 13 papillary thyroid cancer tissues and 4 thyroid cancer cell lines.
  • Utilized reverse transcriptase-polymerase chain reaction (RT-PCR) to analyze RUNX3 gene expression.
  • Treated thyroid cancer cell lines with 5-aza-2'-deoxycytidine (DAC), a demethylating agent, to assess its effect on RUNX3 expression.

Main Results:

  • RUNX3 gene hypermethylation was observed in multiple thyroid cancer cell lines.
  • RUNX3 was hypermethylated in 10 out of 12 papillary thyroid cancer tissue samples.
  • Treatment with DAC led to increased RUNX3 gene expression in some thyroid cancer cell lines.

Conclusions:

  • RUNX3 gene is potentially associated with the carcinogenesis of thyroid cancer.
  • RUNX3 methylation represents a promising diagnostic biomarker for papillary thyroid cancer.
Keywords:
MethylationRUNX3Thyroid neoplasms

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