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Bienzyme-functionalized monodispersed biocompatible cuprous oxide/chitosan nanocomposite platform for biomedical
Jay Singh1, Manish Srivastava, Appan Roychoudhury
1Department of BIN Fusion Technology, Chonbuk National University, Jeonju, Jeonbuk 561-756, Korea.
The Journal of Physical Chemistry. B
|December 29, 2012
Summary
Researchers developed a novel nanocomposite using cuprous oxide nanoparticles and chitosan for a cholesterol biosensor. This biocompatible material shows high sensitivity and stability, enabling accurate cholesterol detection in human serum and demonstrating antimicrobial properties.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Development of sensitive and stable biosensors is crucial for accurate disease diagnosis.
- Cuprous oxide (Cu(2)O) nanoparticles offer unique electrochemical properties for biosensing applications.
- Chitosan (CS) is a biocompatible polymer widely used in biomedical devices and drug delivery systems.
Purpose of the Study:
- To synthesize ultrafine monodispersed cuprous oxide (Ufm-Cu(2)O) nanoparticles and create a Ufm-Cu(2)O-chitosan (CS) nanocomposite.
- To fabricate a novel cholesterol biosensor by immobilizing cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) onto the Ufm-Cu(2)O-CS/indium tin-oxide (ITO) electrode.
- To evaluate the performance, biocompatibility, and antimicrobial activity of the developed nanobiocomposite platform.
Main Methods:
- Facile wet chemical synthesis of Ufm-Cu(2)O nanoparticles using poly-N-vinylpyrrolidone (PVP) capping agent.
- Electrophoretic deposition (EPD) of Ufm-Cu(2)O-CS colloidal solution onto ITO glass substrate.
- Characterization using XRD, SEM, TEM, FTIR; biosensor fabrication and electrochemical performance evaluation; seed germination tests for biocompatibility and antimicrobial activity.
Main Results:
- Successfully synthesized Ufm-Cu(2)O nanoparticles and Ufm-Cu(2)O-CS nanocomposite characterized by various techniques.
- Fabricated a cholesterol biosensor (ChEt-ChOx/Ufm-Cu(2)O-CS/ITO) with linearity from 10-450 mg/dL, a detection limit of 15.9 mg/dL cm(-2), and sensitivity of 0.895 μA/(mg/dL cm(-2)).
- Demonstrated biocompatibility through seed germination tests showing antimicrobial activity against pathogens and crops, along with fast response time (<5 s), good reproducibility, and long-term stability.
Conclusions:
- The Ufm-Cu(2)O-CS nanocomposite serves as a promising biocompatible platform for cholesterol biosensing.
- The developed nanobiocomposite exhibits excellent electrochemical performance and antimicrobial properties, suitable for biomedical applications.
- The biosensor accurately determined total cholesterol levels in human serum samples, highlighting its clinical potential.
