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Updated: May 15, 2026
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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
Published on: March 24, 2023
A new approach to CD34+ hematopoietic progenitor cell counting
Pedro Marín1, Lluís Jover, Jordi Petriz
1Servei d'Hemoteràpia i Hemostàsia, Hospital Clínic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain.
Current Stem Cell Research & Therapy
|December 29, 2012
Summary
Comparing hematopoietic progenitor cell counting methods, a new CD14/CD11b protocol shows validity. Different techniques yield varying results, highlighting the need for standardized progenitor assessment.
Area of Science:
- Hematology
- Immunophenotyping
- Cell Biology
Background:
- Hematopoietic progenitor cell counting lacks a gold standard.
- Significant intra-laboratory variation exists among common assessment strategies.
Purpose of the Study:
- To compare the intra-laboratory variation of commonly used hematopoietic progenitor cell counting strategies.
- To validate a novel CD14/CD11b antibody protocol for progenitor cell assessment.
Main Methods:
- Utilized FITC-conjugated monoclonal antibodies (CD14, CD11b) and PE-CD34 for CD34 counting, excluding CD14(+)/CD11b(+) bright cells.
- Compared the developed protocol against the Milan method (single-staining) and the ISHAGE multiparameter method.
Main Results:
- The CD14/CD11b protocol demonstrated validity for progenitor cell counting.
- Flow cytometry counts showed high concordance (Lin's coefficient), but differed substantially from colony-forming unit counts.
- Both ISHAGE and CD14/CD11b protocols yielded higher counts than the Milan method.
- A rare CD34(+)CD45(neg) cell population was identified, potentially impacting CD34 counts.
Conclusions:
- A valid alternative approach for hematopoietic progenitor cell counting was developed.
- Demonstrated that different progenitor cell counting methods produce varying results.
