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Luman recruiting factor regulates endoplasmic reticulum stress in mouse ovarian granulosa cell apoptosis

Yanzhou Yang1, Pengfei Lin, Fenglei Chen

  • 1Key Laboratory of Animal Biotechnology, Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China.

Theriogenology
|December 29, 2012
PubMed

Insights

Luman recruiting factor (LRF) induces granulosa cell apoptosis via endoplasmic reticulum stress, playing a key role in mouse follicular atresia and selection.

Area of Science:

  • Reproductive Biology
  • Cellular Biology
  • Endocrinology

Background:

  • Follicular atresia is driven by granulosa cell apoptosis, but its molecular triggers are unclear.
  • Understanding granulosa cell apoptosis is crucial for reproductive health and fertility research.

Purpose of the Study:

  • To investigate the role of Luman recruiting factor (LRF), an endoplasmic reticulum stress-responsive gene, in mouse follicular atresia.
  • To elucidate the molecular mechanisms underlying granulosa cell apoptosis during follicular development.

Main Methods:

  • Immunohistochemistry and confocal laser scanning microscopy to localize proteins in granulosa cells.
  • In vitro induction of granulosa cell apoptosis using thapsigargin or tunicamycin.
  • Quantitative analysis of gene expression (mRNA) for LRF and related proteins.

Main Results:

  • LRF protein was detected in the cytoplasm of apoptotic granulosa cells.
  • LRF, Luman, CCAAT/enhancer-binding protein homologous protein, and caspase-12 were upregulated during induced granulosa cell apoptosis.
  • Glucose-regulated protein 78 was present only in healthy granulosa cells, suggesting a protective role.

Conclusions:

  • LRF is implicated in inducing granulosa cell apoptosis through the endoplasmic reticulum stress pathway.
  • LRF may play a critical role in the selection process of ovarian follicles in mice.

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