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Updated: May 15, 2026

Measuring Peptide Translocation into Large Unilamellar Vesicles
Published on: January 27, 2012
Peptide translocation through the mesoscopic channel: binding kinetics at the single molecule level
Usha Lamichhane1, Tuhidul Islam, Sonal Prasad
1School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759, Bremen, Germany.
Abstract:
Single channel electrophysiological studies have been carried out to elucidate the underlying interactions during the translocation of polypeptides through protein channels. For this we used OmpF from the outer cell membrane of E. coli and arginine-based peptides of different charges, lengths and covalently linked polyethylene glycol as a model system. In order to reveal the fast kinetics of peptide binding, we performed a temperature scan. Together with the voltage-dependent single-channel conductance, we quantify peptide binding and translocation.
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