New bacteria Bacillus nitroreducens PLC9 with hydrogen peroxide-degrading activity with high survival rate in...

Bacteria were isolated from wastewater containing highly concentrated hydrogen peroxide that had been used to clean the pure water delivery system in a semiconductor plant. One bacterium was selected for its high hydrogen peroxide degradation activity. In the presence of 1% hydrogen peroxide, it degraded 72.5% in 5 min. It showed 100% viability after 6 h at 1% hydrogen peroxide. Even at 3% hydrogen peroxide, it survived for more than 6 h. This bacterium was named as Bacillus nitroreducens PLC9 since its 16S rRNA showed 100% similarity with the recently reported new species B. nitroreducens. Purified catalase from B. nitroreducens PLC9 was characterized as a thermo-alkali-stable hydroperoxidase type II catalase, and it is suggested as a new type of catalase based on following: (1) it is stable over a broad pH range (pH 4-11); (2) it is consisted of homodimers with a molecular weight of 66 kDa (total molecular weight, 134 kDa); (3) its activity was not inhibited by 3-amino-1,2,4-triazole; and (4) its N-terminal sequence has never been reported before. Both B. nitroreducens PLC9 and the isolated catalase can be used for efficient degradation of hydrogen peroxide at high concentrations.