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Related Concept Videos

DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
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Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
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Replication in Eukaryotes01:29

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In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
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Replication in Prokaryotes01:32

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DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
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Related Experiment Video

Updated: May 15, 2026

The Participant-Reported Implementation Update and Score (PRIUS): A Novel Method for Capturing Implementation-Related Data Over Time
06:05

The Participant-Reported Implementation Update and Score (PRIUS): A Novel Method for Capturing Implementation-Related Data Over Time

Published on: February 19, 2021

Implementing evidence-based programs: a four-step protocol for assuring replication with fidelity.

Michiyo Tomioka1, Kathryn L Braun

  • 11Myron B. Thompson School of Social Work, University of Hawaii at Mānoa, Honolulu, HI, USA.

Health Promotion Practice
|December 29, 2012
PubMed
Summary
This summary is machine-generated.

Replicating evidence-based health programs requires a structured approach. This protocol ensures key components are delivered with fidelity for successful community adaptation and improved health outcomes in older adults.

Keywords:
community planningevaluationevidence-based interventionfidelity monitoringhealth promotionorganizational capacitypartnership development

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Area of Science:

  • Public Health
  • Health Promotion
  • Gerontology

Background:

  • Health care providers face challenges in replicating evidence-based programs.
  • Adapting programs for new communities requires maintaining fidelity of key components.

Purpose of the Study:

  • To describe a four-step fidelity assurance protocol for adapting and replicating evidence-based health promotion programs.
  • To guide communities in successful implementation of public health initiatives for older adults.

Main Methods:

  • Deconstruct programs into components and create replication plans.
  • Identify suitable agencies and provide staff training.
  • Monitor program delivery fidelity using standardized checklists.
  • Track participant outcomes to ensure expected results.

Main Results:

  • The Hawai'i's Healthy Aging Partnership successfully replicated the EnhanceFitness program using the protocol.
  • The protocol demonstrated effectiveness in maintaining program fidelity and achieving outcomes.

Conclusions:

  • The described four-step fidelity assurance protocol is transferable to other communities.
  • This protocol supports the successful adaptation and replication of evidence-based public health programs.