Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Buruli Ulcer-Associated Staphylococcus aureus in Burkina Faso.

The American journal of tropical medicine and hygiene·2026

Same author

Evidence of a millennia-old association between a methanogenic archaeon and a bacterium in dental calculus: A re-analysis of ancient and modern metagenomic datasets.

Archives of oral biology·2026

Same author

Retraction Note: Screening Strategy of Active Pulmonary Tuberculosis in Sheltered Homeless People in Marseille, 2019.

Journal of epidemiology and global health·2026

Same author

Retraction Note: Preliminary Feasibility Study of Questionnaire-based Active Pulmonary Tuberculosis Screening in Marseille Sheltered Homeless People, Winter 2018.

Journal of epidemiology and global health·2026

Same author

Snapshot based on whole-genome sequencing revealing the species and antimicrobial susceptibility profiles of Mycobacterium tuberculosis complex recovered at a major tertiary care center in Lebanon.

BMC infectious diseases·2026

Same author

Commentary on Seersholm Et al.: Yersinia pestis Infection Is Not Synonymous With Deadly Plague in Neolithic Scandinavia.

American journal of biological anthropology·2026

Same journal

Feasibility of delivering a digital multiple health behaviour intervention via a home-telemonitoring system: engagement, outcomes, and user experiences.

BMC research notes·2026

Same journal

From manual entry to machine precision: challenges and evolution of metadata schema development in collaborative research centers.

BMC research notes·2026

Same journal

Sleep deprivation impairs gastric ulcer healing and induces anxiety-like behavior in rats.

BMC research notes·2026

Same journal

Caring across cultures: a qualitative exploration of Macedonian carers supporting community-dwelling older adults in a large regional city in Australia.

BMC research notes·2026

Same journal

Prevalence and determinants of dyslipidemia among residents of Kurdistan Region of Iraq; a community-based study.

BMC research notes·2026

Same journal

Modulation of brain-kidney crosstalk by olanzapine in aluminum chloride-induced memory impairment: a preclinical investigation.

BMC research notes·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 8, 2026

Extraction of High Molecular Weight DNA from Microbial Mats

Extraction of High Molecular Weight DNA from Microbial Mats

Published on: July 7, 2011

Optimized microbial DNA extraction from diarrheic stools.

Emilie Donatin¹, Michel Drancourt

¹Aix Marseille Université, URMITE, UMR63 CNRS 7278, IRD 198, Inserm 1095, 13005, Marseille, France.

BMC Research Notes

|January 1, 2013

Summary

This summary is machine-generated.

Lyophilization of diarrheic stool specimens significantly enhances the PCR-based detection of microorganisms. This improved protocol aids in the molecular diagnosis of infectious diarrhea.

More Related Videos

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

Related Experiment Videos

Last Updated: Apr 8, 2026

Extraction of High Molecular Weight DNA from Microbial Mats

Extraction of High Molecular Weight DNA from Microbial Mats

Published on: July 7, 2011

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

Area of Science:

Microbiology
Molecular Biology
Clinical Diagnostics

Background:

Nucleic acid-based detection is crucial for identifying enteropathogens in stool.
Detection can be challenging in diarrheic stool specimens.
An improved protocol involving stool lyophilization and semi-automated DNA extraction was developed.

Purpose of the Study:

To evaluate the effectiveness of stool lyophilization combined with semi-automated DNA extraction for enhancing pathogen detection.
To assess the impact of lyophilization on the PCR-based detection of bacteria and specific pathogens in diarrheic stool samples.

Main Methods:

41 human diarrheic stool specimens were analyzed.
Specimens were divided into lyophilized and non-lyophilized aliquots.
DNA was extracted using a combination of automated and phenol-chloroform methods.
Real-time PCR was performed to detect Bacteria (16S rRNA), Methanobrevibacter smithii, and Salmonella enterica.

Main Results:

Lyophilization significantly increased the detection rates for M. smithii (63.4% to 95.1%) and Bacteria (80.5% to 97.6%).
Detection rates for S. enterica remained high (100%) in both lyophilized and non-lyophilized samples.
Lyophilization led to significantly higher proportions of positive specimens for M. smithii (p=0.00043) and Bacteria (p=0.015).

Conclusions:

Lyophilization of diarrheic stool specimens substantially improves PCR-based detection of microorganisms.
The described semi-automated protocol is suitable for routine molecular diagnosis of infectious diarrhea.