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Related Experiment Video

Updated: May 15, 2026

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

Foxa2 may modulate hepatic apoptosis through the cIAP1 pathway.

Kewei Wang¹, John J Brems, Richard L Gamelli

¹Departments of Surgery, University of Illinois College of Medicine at Peoria, IL 61605, USA. kewang@uic.edu

Cellular Signalling

|January 1, 2013

Summary

The liver transcription factor Foxa2 (forkhead box A2) protects against liver injury by inhibiting hepatocyte apoptosis. Foxa2 regulates the anti-apoptotic gene cIAP1, offering a therapeutic target for liver diseases.

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Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

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Published on: June 29, 2011

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Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

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Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Published on: June 29, 2011

Area of Science:

Hepatology
Molecular Biology
Cell Death Research

Background:

Hepatocyte apoptosis is a key feature of chronic liver injury.
The precise molecular mechanisms driving this apoptosis are not fully understood.
The liver-enriched transcription factor Foxa2 (forkhead box A2) is linked to inflammation and neoplasia and may regulate apoptosis.

Purpose of the Study:

To investigate the relationship between Foxa2 and hepatic apoptosis.
To elucidate the molecular mechanisms by which Foxa2 influences liver cell death.

Main Methods:

Induction of apoptosis in liver cells using various factors.
Measurement of caspase activity and TUNEL assay to quantify apoptosis.
Analysis of Foxa2 expression, gene silencing (siRNA), and overexpression (vectors) in HepG2 cells.
Correlation of Foxa2 levels with anti-apoptotic gene expression (cIAP1, cIAP2, XIAP, survivin).
Electrophoretic mobility shift assay (EMSA), gel supershift assay, and chromatin immunoprecipitation (ChIP) assay to assess Foxa2 binding to the cIAP1 promoter.

Main Results:

Apoptotic liver injury correlated with decreased Foxa2 expression.
Foxa2 overexpression reduced apoptosis, while Foxa2 silencing increased apoptosis in HepG2 cells.
Foxa2 expression levels were associated with anti-apoptotic genes, notably cIAP1.
Foxa2 directly binds to the cIAP1 promoter, regulating its activity and expression.

Conclusions:

Foxa2 transcription factor modulates hepatic apoptosis potentially via the cIAP1 signaling pathway.
Foxa2 represents a potential therapeutic target for treating liver diseases characterized by apoptosis.