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Related Concept Videos

Indicators02:39

Indicators

Certain organic substances change color in dilute solution when the hydronium ion concentration reaches a particular value. For example, phenolphthalein is a colorless substance in any aqueous solution with a hydronium ion concentration greater than 5.0 × 10−9 M (pH < 8.3). In more basic solutions where the hydronium ion concentration is less than 5.0 × 10−9 M (pH > 8.3), it is red or pink. Substances such as phenolphthalein, which can be used to determine the pH of a solution, are called...
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Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
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Disposable fluorescence optical pH sensor for near neutral solutions.

Luca Ferrari1, Luigi Rovati, Paola Fabbri

  • 1Department of Engineering Enzo Ferrari, University of Modena and Reggio Emilia,via Vignolese 905, Modena I-41125, Italy. luca.ferrari@unimore.it

Sensors (Basel, Switzerland)
|January 1, 2013
PubMed
Summary

A novel fluorescence-based pH sensor using a fluorescein dye in a hydrogel matrix offers accurate, on-line measurements for near-neutral solutions. This low-cost, disposable sensor demonstrates excellent linearity and stability for reliable pH monitoring.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Accurate pH monitoring is crucial in various scientific and industrial applications.
  • Existing pH sensors may have limitations in terms of cost, disposability, or suitability for specific solution types.
  • Development of novel sensing platforms is needed for improved on-line pH measurements.

Purpose of the Study:

  • To design, develop, and evaluate a fluorescence-based pH sensor for on-line measurements.
  • To create a low-cost, disposable sensor suitable for near-neutral solutions.
  • To characterize the sensor's performance, including its pKa, operating range, linearity, drift, response time, and reproducibility.

Main Methods:

  • A fluorescence-based pH sensor was developed using fluorescein O-methacrylate covalently linked to a hydrogel matrix (HEMA, HDDA, PEGDA).
  • An optoelectronic transduction system was designed for interrogating the polymer sensing probe and processing the fluorescence signal.
  • Performance evaluation included determining the pKa, operational pH range, linearity, short-term drift, response time, and reproducibility.

Main Results:

  • The sensor's pKa was determined to be 7.9, making it suitable for near-neutral solutions.
  • The sensor operates effectively within a pH range of 6.5 to 9.0, with linear behavior between pH 7.0 and 8.0 (max linearity error of 5%).
  • Short-term drift was less than 0.15% over 40 minutes, demonstrating good stability, along with good response time and reproducibility.

Conclusions:

  • The developed fluorescence-based pH sensor is a viable tool for on-line monitoring of near-neutral solutions.
  • The sensor offers a cost-effective and disposable solution with high performance in terms of accuracy, stability, and reproducibility.
  • This technology has potential applications in various fields requiring precise pH measurements.