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Published on: September 13, 2018

Deletion of leucine zipper tumor suppressor 2 (Lzts2) increases susceptibility to tumor development

Daniel T Johnson1, Richard Luong, Suk Hyung Lee

  • 1Department of Urology, Stanford University School of Medicine, Stanford, California 94305-5328, USA.

The Journal of Biological Chemistry
|January 1, 2013
PubMed

Insights

Loss of the Lzts2 gene in mice increases tumor development. This study demonstrates that Lzts2 deficiency promotes both spontaneous and carcinogen-induced tumor formation, highlighting its role in cancer prevention.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • The LZTS2 protein's function in tumor suppression is not fully understood.
  • Genetic alterations in LZTS2 may contribute to cancer development.

Purpose of the Study:

  • To investigate the biological role of Lzts2 in tumorigenesis using a mouse model.
  • To determine the impact of Lzts2 deletion on spontaneous and carcinogen-induced tumor development.

Main Methods:

  • Generation and characterization of Lzts2 knock-out mouse models (heterozygous and homozygous).
  • Administration of N-butyl-N-(4-hydroxybutyl) nitrosamine to induce bladder carcinoma.
  • Analysis of LZTS2 protein expression in human prostate cancer tissues.
  • Assessment of cell growth in mouse embryonic fibroblasts derived from Lzts2 knock-out embryos.

Main Results:

  • Lzts2 deletion, both heterozygous and homozygous, significantly increased spontaneous tumor incidence in mice.
  • Homozygous Lzts2 knock-out mice exhibited a higher tumor incidence than heterozygous mice.
  • Lzts2-deficient mice showed increased susceptibility to carcinogen-induced bladder carcinoma.
  • Reduced LZTS2 protein expression was observed in human prostate cancer tissues.
  • Loss of Lzts2 enhanced cell growth in mouse embryonic fibroblasts.

Conclusions:

  • Lzts2 acts as a tumor suppressor, and its deletion increases susceptibility to tumor development.
  • The absence of Lzts2 promotes both spontaneous and carcinogen-induced tumorigenesis.
  • LZTS2 deficiency may play a role in human prostate cancer progression.

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