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Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 15, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

PRASA: an integrated web server that analyzes protein interaction types.

Chen-Yu Fan1, Yi-Han Bai, Cheng-Yi Huang

  • 1Department of Electrical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.

Gene
|January 2, 2013
PubMed
Summary
This summary is machine-generated.

The Protein Association Analyzer (PRASA) tool distinguishes between protein interaction types, crucial for understanding biological functions. It uses compiled data to build models, offering insights into complex protein roles.

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Identifying Protein-protein Interaction Sites Using Peptide Arrays
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09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Molecular Biology

Background:

  • Protein interactions are fundamental to biological processes.
  • Distinguishing diverse interaction types (e.g., physical contact, functional relation) is complex.
  • Existing tools often fail to differentiate interaction types, leading to confusion.

Purpose of the Study:

  • To present the Protein Association Analyzer (PRASA), a novel tool for predicting protein interactions and their specific types.
  • To address the limitations of existing tools by differentiating between various interaction types.
  • To provide a centralized platform for analyzing and comparing protein interaction types.

Main Methods:

  • Compiled a dataset of 7,234,058 experimentally verified protein associations from five databases.
  • Developed individual probabilistic models for distinct protein interaction types.
  • Integrated these models into the PRASA web platform.

Main Results:

  • PRASA successfully predicts protein associations and categorizes them by interaction type.
  • The tool's results page displays predicted associations with supporting references, organized by interaction type.
  • Experimental validation confirmed the performance benefits of distinguishing between different interaction types.

Conclusions:

  • PRASA offers a significant advancement in analyzing protein interactions by differentiating types.
  • The platform facilitates browsing, downloading, and comparing interaction data, aiding biological research.
  • PRASA enhances understanding of the complex roles proteins play in biological systems.