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Related Concept Videos

Transgenic Organisms00:53

Transgenic Organisms

Overview
Transgenic Organisms00:53

Transgenic Organisms

Overview
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Complementation Tests00:49

Complementation Tests

A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: May 15, 2026

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
11:48

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Published on: January 17, 2016

Transgenic chickens.

Ken-ichi Nishijima1, Shinji Iijima

  • 1Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan. nishijma@nubio.nagoya-u.ac.jp

Development, Growth & Differentiation
|January 3, 2013
PubMed
Summary

Recent advancements enable transgenic chicken development, overcoming previous limitations in obtaining suitable cells and microscopic observation. These new methods position chickens as a valuable genetic research model.

Area of Science:

  • Animal Biotechnology
  • Developmental Biology
  • Genetics

Background:

  • Transgenic chicken technology development has historically lagged behind mammalian systems.
  • Challenges include obtaining suitable one-cell-stage oocytes and yolk interference with microscopic observation.
  • These limitations have hindered the efficient creation of genetically modified chickens.

Purpose of the Study:

  • To review recent advancements in transgenic chicken technology.
  • To highlight novel methods overcoming previous technical barriers.
  • To assess the potential of chickens as a model genetic system.

Main Methods:

  • Utilizing retroviral vectors for efficient transgene integration into host cell chromosomes.
  • Direct injection of viral vectors into blastodermal-stage embryos.

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Last Updated: May 15, 2026

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
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Published on: January 17, 2016

Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model
09:23

Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model

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  • In vitro infection of primordial germ cells (PGCs) followed by implantation into recipient embryos.
  • Long-term culture of PGCs for selection of transfected cells and subsequent implantation.
  • Exploration of embryonic stem (ES) cell systems, though functional gamete induction remains a challenge.

    • Main Results:

    • Several new methods have been successfully developed for establishing transgenic chickens.
    • Retroviral vectors demonstrate high efficiency in transgene incorporation.
    • Alternative methods, including PGC manipulation and ES cell systems, offer further avenues.
    • Despite challenges with ES cell-derived gametes, overall progress is significant.

    Conclusions:

    • Recent technological developments have significantly advanced the feasibility of creating transgenic chickens.
    • Chickens are poised to become a valuable experimental genetic system due to these breakthroughs.
    • Continued research into PGC and ES cell manipulation will further refine transgenic chicken production.