Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interaction between prostasomes and spermatozoa from human semen.

G Ronquist1, B O Nilsson, S Hjertën

  • 1Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.

Archives of Andrology
|January 1, 1990
PubMed
Summary

Prostasomes, prostate-derived organelles, interact with spermatozoa via hydrophobic bonds, enhancing sperm motility. This interaction is crucial for creating a favorable microenvironment for sperm function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quadriceps and hamstring muscle activity during cycling as measured with intramuscular electromyography.

European journal of applied physiology·2016
Same author

1α,25-dihydroxyvitamin D3 promotes osteogenic activity and downregulates proinflammatory cytokine expression in human periodontal ligament cells.

Journal of periodontal research·2014
Same author

High levels of cathepsins B, L and S in human seminal plasma and their association with prostasomes.

Andrologia·2012
Same author

The inverted CD4/CD8 ratio and associated parameters in 66-year-old individuals: the Swedish HEXA immune study.

Age (Dordrecht, Netherlands)·2012
Same author

Prostasomes are mediators of intercellular communication: from basic research to clinical implications.

Journal of internal medicine·2011
Same author

Ischaemic pre-conditioning means an increased adenosine metabolism with decreased glycolytic flow in ischaemic pig myocardium.

Acta anaesthesiologica Scandinavica·2010

Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Biochemistry

Background:

  • Prostasomes are organelles from the prostate found in seminal plasma.
  • They are known to enhance sperm forward motility through an unclear mechanism.
  • Understanding prostasome-spermatozoa interaction is key to reproductive health.

Purpose of the Study:

  • To investigate the interaction mechanism between human prostasomes and spermatozoa.
  • To elucidate the nature of the bonds facilitating prostasome-spermatozoa association.
  • To confirm the physical interaction and its implications for sperm function.

Main Methods:

  • Free-zone electrophoresis was used to observe the behavior of prostasomes and spermatozoa mixtures.
  • Transmission electron microscopy (TEM) was employed to visualize the interaction.

Related Experiment Videos

  • Surface charge analysis and neuraminidase treatment were performed to probe interaction characteristics.

    • Main Results:

    • Prostasomes and spermatozoa, despite both having negative surface charges, fused into a single stable peak during electrophoresis.
    • TEM confirmed the co-localization and interaction of prostasomes with spermatozoa.
    • Interaction was observed with hydrophilic resin embedding, suggesting hydrophobic bonding.

    Conclusions:

    • Human prostasomes and spermatozoa interact strongly through hydrophobic forces.
    • This hydrophobic interaction is essential for prostasomes to create a supportive microenvironment for sperm motility.
    • The findings provide insight into the functional role of prostasomes in male fertility.