Related Experiment Video
Updated: May 15, 2026
Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
Published on: May 7, 2019
Inhibition of fibroblast proliferation in vitro using low-level infrared light-emitting diodes
Hadar Lev-Tov1, Neil Brody, Daniel Siegel
1Department of Dermatology, University of California at Davis, Sacramento, California, USA.
Background:
Scars, including hypertrophic and keloidal-type scars, may occur after burns, trauma, or surgery. Despite several treatment options available for scars, few effective, noninvasive modalities exist. Recently, a few small clinical studies revealed the possible benefit of red and infrared (IR) low-level light therapy (LLLT) in scar treatment. One of the important features of scars is proliferation of dermal fibroblasts, but in vitro data regarding the effects of light-emitting diode (LED)-generated IR light on human skin fibroblasts is lacking.
Objective:
To evaluate the effect of IR LLLT generated using LEDs on fibroblast proliferation and viability in vitro.
Methods And Materials:
Irradiation of normal human skin fibroblasts using IR LED panels was performed in vitro, and modulation of proliferation and viability was quantified using Trypan blue dye exclusion assay.
Results:
Fluences of 80, 160 and 320 J/cm(2) resulted in statistically significantly less fibroblast proliferation than in controls, without statistically significantly less cellular viability.
Conclusion:
IR LLLT can effectively inhibit fibroblast proliferation in vitro without altering viability and holds promise for the treatment of scars.
More Related Videos
12:35Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
Published on: April 14, 2023
07:36Clinical Application of Intense Pulsed Light Therapy and Radio Frequency for Treatment of Ocular Surface Diseases
Published on: July 3, 2025