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Reproductive Cloning

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...

Reproductive Cloning

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...

Cloning of Dolly the Sheep

Cloning of Dolly the Sheep

The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual, the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter telomeres than other...

Introduction to Nuclear Reprogramming

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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...

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Updated: May 15, 2026

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

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Published on: July 6, 2022

Species-specific challenges in dog cloning.

G A Kim¹, H J Oh, J E Park

¹Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.

Reproduction in Domestic Animals = Zuchthygiene

|January 3, 2013

Summary

Somatic cell nuclear transfer (SCNT) optimizes dog cloning by analyzing key steps from nuclear removal to recipient transfer. This review identifies crucial factors for improving canine cloning success rates.

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Area of Science:

Reproductive Biology
Animal Cloning
Genetics

Background:

Somatic cell nuclear transfer (SCNT) is a widely used technique for cloning various animal species.
Dog cloning involves intricate steps: nuclear material removal, donor cell injection, fusion, oocyte activation, and embryo transfer.
Numerous factors influence the overall efficiency of canine cloning.

Purpose of the Study:

To define the optimal procedure for dog cloning.
To summarize specific features that enhance canine cloning efficiency.
To provide a comprehensive overview based on published literature from 2005-2012.

Main Methods:

Retrospective analysis of published dog cloning research.
Review of papers published between 2005 and 2012.
Identification and synthesis of critical procedural steps and influencing factors.

Main Results:

Detailed breakdown of the SCNT process in dogs, highlighting critical stages.
Identification of key variables impacting cloning success.
Summary of best practices and specific considerations for canine SCNT.

Conclusions:

The optimal dog cloning procedure is defined by meticulous execution of each SCNT step.
Understanding specific features of canine reproductive biology is essential for efficiency.
This review consolidates knowledge to guide future dog cloning efforts.