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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

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Published on: July 7, 2020

Metallo-beta-lactamase producing gram-negative bacterial isolates.

S K Mishra¹, J Acharya, H P Kattel

¹Department of Microbiology, Institute of Medicine, TUTH, Kathmandu, Nepal. smishra7@hotmail.com

Journal of Nepal Health Research Council

|January 3, 2013

Summary

Metallo-β-lactamase (MBL) producing gram-negative bacteria were detected in lower respiratory tract infections in Nepal. This study provides crucial baseline data on MBL prevalence in the region.

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Area of Science:

Microbiology
Infectious Diseases
Antimicrobial Resistance

Background:

Global rise of metallo-β-lactamase (MBL) producing bacteria poses treatment challenges for lower respiratory tract infections (LRTI).
Limited data exists on MBL prevalence in Nepal.
This study addresses the knowledge gap regarding MBL-producing isolates in Nepal.

Purpose of the Study:

To determine the prevalence of metallo-β-lactamase (MBL) producing bacterial isolates from lower respiratory tract infections (LRTI) in Nepal.
To establish baseline data on MBL-producing bacteria in the Nepalese context.

Main Methods:

Cross-sectional study conducted over six months (June-November 2008) at a teaching hospital.
1120 lower respiratory tract specimens (sputum, endotracheal secretion, bronchial washing) analyzed.
Combination disk (CD) and Double disk synergy test (DDST) methods used for MBL detection.

Main Results:

Respiratory pathogens found in 44.4% of suspected LRTI cases.
Gram-negative bacteria constituted 84.0% of isolates.
Metallo-β-lactamase (MBL) was detected in 6 (1.3%) of 448 gram-negative isolates, specifically in Pseudomonas aeruginosa and Acinetobacter spp.
All MBL-producing isolates were multidrug-resistant (MDR).

Conclusions:

Metallo-β-lactamase (MBL) producing gram-negative bacteria were identified in lower respiratory tract infection isolates in Nepal.
This study provides essential baseline data on MBL-producing bacteria in the region.
Findings highlight the emergence of MBL-producing pathogens, necessitating further surveillance and therapeutic strategy development.