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Related Experiment Videos

Hexokinase redistribution in vivo.

S E Laursen1, J K Belknap, K E Sampson

  • 1Department of Anatomy and Cell Biology, University of North Dakota, Grand Forks 58202.

Biochimica Et Biophysica Acta
|April 23, 1990
PubMed
Summary

Alcohol affects brain hexokinase (HK) distribution differently in seizure-prone and seizure-resistant mice. This enzyme redistribution may explain varying alcohol withdrawal seizure sensitivities.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Pharmacology

Background:

  • Hexokinase (HK) is crucial for glucose metabolism in the brain.
  • Alcohol withdrawal seizures are a significant clinical concern.
  • Genetic differences in mice (WSR and WSP) exhibit varying seizure severities.

Purpose of the Study:

  • To investigate the role of brain hexokinase (HK) distribution in alcohol withdrawal seizure severity.
  • To compare HK responses to alcohol in seizure-resistant (WSR) and seizure-prone (WSP) mice.

Main Methods:

  • Utilized heterogeneous stock mice and selectively bred WSR and WSP mice.
  • Administered acute alcohol doses (4 g/kg) and saline controls.
  • Measured changes in cytosolic hexokinase (HK) concentration in cerebellum and cerebrum under ischemic conditions and after alcohol administration.

Main Results:

  • Cerebellar and cerebral cytosolic HK concentrations decreased during ischemic responses post-mortem.
  • WSR mice showed an 18.5% decrease in cytosolic HK after alcohol administration.
  • WSP mice exhibited a 20.3% increase in cytosolic HK post-alcohol, which normalized under prolonged ischemia.

Conclusions:

  • Alcohol induces a redistribution of hexokinase (HK) in the brain in vivo.
  • Altered HK distribution in WSP mice may contribute to their increased susceptibility to alcohol-related seizures.
  • HK redistribution is a potential mechanism underlying differential alcohol sensitivity and seizure risk.

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