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Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: May 15, 2026

Gene Trapping Using Gal4 in Zebrafish
13:34

Gene Trapping Using Gal4 in Zebrafish

Published on: September 29, 2013

Trapping cardiac recessive mutants via expression-based insertional mutagenesis screening.

Yonghe Ding1, Weibin Liu, Yun Deng

  • 1Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.

Circulation Research
|January 4, 2013
PubMed
Summary

We developed a gene trapping method in zebrafish to efficiently identify cardiac mutants, including those with adult heart conditions. This approach simplifies screening for genes affecting heart development and function.

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Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

Area of Science:

  • Developmental Biology
  • Genetics
  • Cardiovascular Research

Background:

  • Mutagenesis screening is vital for understanding vertebrate development and disease.
  • Managing vertebrate colonies for genetic screens, especially for adult phenotypes, is challenging.
  • Identifying genes affecting specific organs like the heart requires efficient screening methods.

Purpose of the Study:

  • To develop a streamlined method for managing colonies and screening for cardiac mutants.
  • To facilitate the identification of genes with adult phenotypes affecting the heart.
  • To enrich for cardiac mutants for efficient adult phenotype screening.

Main Methods:

  • Utilized a transposon-based protein trapping vector for insertional mutagenesis in zebrafish.
  • Screened 67 stable transgenic lines for cardiac monomeric red fluorescent protein reporter expression.
  • Defined molecular identities of 10 lines and bred them to homozygosity to identify lethal and adult mutants.

Main Results:

  • Identified 15 transgenic lines with cardiac reporter expression.
  • Discovered 1 embryonic lethal, 1 larval lethal, and 1 adult recessive mutant with cardiac hypertrophy.
  • Uncovered roles for mat2aa in cardiogenesis, mrps18b in cardiac mitochondrial homeostasis, and dnjab6b in adult cardiac hypertrophy.

Conclusions:

  • Transposon-based gene trapping efficiently identifies embryonic and adult recessive cardiac mutants in zebrafish.
  • This zebrafish mutant collection aids in annotating the vertebrate cardiac genome.
  • Enables heart-based adult screens for novel genetic discoveries.