Related Experiment Video
Updated: May 15, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Common screening approaches for efficient analytical method development in LC and SFC on columns packed with
1Chiral Technologies Europe, Illkirch, France. pfranco@chiral.fr
Abstract:
Owing to their remarkable enantioselectivity, versatility, and stability, immobilized polysaccharide-based chiral stationary phases (CSPs) have been successfully integrated into the tool box of many research and industry groups for the separation of enantiomers or stereoisomers by liquid and supercritical fluid chromatography. Due to the structurally diverse range of compounds available, efficient method development for chiral separations utilizing such CSPs is a challenging subject. In this chapter, we will discuss simplified screening protocols and straightforward approaches to achieve chiral separations in HPLC and SFC using the column series CHIRALPAK™ IA, IB, IC, and ID in reasonable time frame and with limited experimental work and a high success rate.
Related Concept Videos
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...
Ion-Exchange Chromatography
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
