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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...

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Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
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Chiral separations by simulated moving bed method using polysaccharide-based chiral stationary phases.

Toshiharu Minoda1

  • 1Daicel Corporation, Niigata, Japan. to_minoda@jp.daicel.com

Methods in Molecular Biology (Clifton, N.J.)
|January 4, 2013
PubMed
Summary

Continuous chiral separation using simulated moving bed (SMB) chromatography is preferred for industrial production over batch methods. This chapter details practical applications of SMB with polysaccharide-derived chiral stationary phases (CSPs).

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Area of Science:

  • Chemical Engineering
  • Separation Science
  • Chromatography

Background:

  • Industrial-scale chromatography often requires continuous separation methods for improved productivity and reduced solvent usage.
  • Batch separation processes in chromatography can be inefficient for large-scale production.
  • Simulated Moving Bed (SMB) chromatography is a widely adopted continuous separation technique.

Purpose of the Study:

  • To introduce practical applications of chiral simulated moving bed (SMB) chromatography.
  • To showcase the use of polysaccharide-derived chiral stationary phases (CSPs) in SMB.
  • To present laboratory-scale separation data for chiral SMB processes.

Main Methods:

  • Simulated Moving Bed (SMB) chromatography.
  • Utilization of polysaccharide-derived chiral stationary phases (CSPs).
  • Laboratory-scale experimental setup and data collection.

Main Results:

  • Demonstration of effective chiral separations using SMB.
  • Successful application of polysaccharide-derived CSPs in continuous chromatographic processes.
  • Presentation of relevant separation data from laboratory-scale experiments.

Conclusions:

  • Continuous chiral separation via SMB is a viable and efficient industrial process.
  • Polysaccharide-derived CSPs are suitable for chiral SMB applications.
  • The presented data supports the practical implementation of chiral SMB.