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Published on: December 23, 2016
Cyclodextrin-modified micellar electrokinetic chromatography for enantioseparations
Wan Aini Wan Ibrahim1, Dadan Hermawan, Mohd Marsin Sanagi
1Separation Science and Technology Group (SepSTec), Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor, Malaysia. wanaini@kimia.fs.utm.my
This study demonstrates cyclodextrin-modified micellar electrokinetic chromatography for separating enantiomers. The method effectively resolves chiral triazole fungicides and econazole, crucial for pharmaceutical and agrochemical analysis.
Area of Science:
- Analytical Chemistry
- Chiral Separations
- Capillary Electrophoresis
Background:
- Enantiomer separation is vital in pharmaceuticals and agrochemicals due to stereochemistry's impact on biological activity.
- Cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) is a key technique for chiral separations.
- Hydroxypropyl-γ-cyclodextrin and sodium dodecyl sulfate are effective components in CD-MEKC.
Purpose of the Study:
- To develop and showcase a CD-MEKC method for enantioseparation.
- To validate the method's efficacy for specific agrochemical and pharmaceutical compounds.
Main Methods:
- Utilizing capillary electrophoresis with cyclodextrin-modified micelles.
- Employing hydroxypropyl-γ-cyclodextrin as the chiral selector.
- Using sodium dodecyl sulfate as the micellar solution.
Main Results:
- Successful enantioseparation of triazole fungicides.
- Effective chiral resolution of the drug econazole.
- Demonstration of CD-MEKC's capability for complex enantiomeric mixtures.
Conclusions:
- The described CD-MEKC method provides a robust approach for enantioseparation.
- This technique is valuable for quality control in the pharmaceutical and agrochemical industries.
- Hydroxypropyl-γ-cyclodextrin offers excellent chiral recognition capabilities.
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