Related Experiment Video
Updated: May 15, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
Global effect of inauhzin on human p53-responsive transcriptome
Jun-Ming Liao1, Shelya X Zeng, Xiang Zhou
1Department of Biochemistry & Molecular Biology and Tulane Cancer Center, Tulane University School of Medicine, New Orleans, Louisiana, USA.
Inauhzin (INZ) globally impacts the p53-responsive transcriptome, activating numerous p53 target genes involved in critical cellular processes like apoptosis and cell cycle regulation. This study identifies potential novel p53 targets and informs future Inauhzin clinical trials.
Area of Science:
- Molecular biology
- Cancer research
- Genomics
Background:
- Inauhzin (INZ) previously demonstrated tumor suppression by inhibiting Sirt1 and activating p53.
- The global impact of INZ on p53-dependent gene expression remained uncharacterized.
Purpose of the Study:
- To determine the global effect of Inauhzin (INZ) on the human p53-responsive transcriptome.
- To identify novel p53 target genes regulated by INZ.
Main Methods:
- Whole genome microarray analysis in HCT116(p53+/+) and HCT116(p53-/-) cells treated with Inauhzin (INZ).
- Real-time quantitative PCR (RT-qPCR) for gene expression validation.
- Gene Ontology (GO) and pathway analyses.
Main Results:
- Inauhzin (INZ) treatment resulted in significant p53-dependent up-regulation (324 genes) and down-regulation (266 genes) of gene expression.
- Affected biological processes include apoptosis, cell cycle, immune system process, and cell adhesion.
- ACBD4, APOBEC3C, and FLJ14327 were identified as potential novel p53 target genes.
Conclusions:
- Inauhzin (INZ) globally induces p53 target gene expression, confirming its activation of the p53 signaling pathway.
- Findings provide insights into novel Inauhzin (INZ) and p53 targets.
- The global transcriptome effects of Inauhzin (INZ) can guide future clinical trial designs.
More Related Videos
09:32Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
Published on: September 10, 2017
08:35Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
Published on: January 4, 2012
Related Concept Videos
Abnormal Proliferation
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein.
The Intrinsic Apoptotic Pathway
Epigenetic Regulation
X-chromosome...