A simple method for demonstrating the giardia lamblia trophozoite.
Monali N Rajurkar1, Niharika Lall, Silpi Basak
1Tutor, Department of Microbiology, Jawaharlal Nehru Medical College, Wardha (M.S.) - 442004, India.
Journal of Clinical and Diagnostic Research : JCDR
|January 4, 2013
Summary
A new methylene blue staining method effectively detects Giardia lamblia trophozoites in stool samples. This simple, permanent staining technique aids in diagnosing giardiasis, a common intestinal infection.
Area of Science:
- Medical Parasitology
- Diagnostic Microbiology
Background:
- Giardia lamblia is a flagellated protozoan parasite causing giardiasis.
- Infection occurs through ingestion of cyst-contaminated water or food.
- Symptoms include diarrhea, flatulence, and steatorrhea.
Purpose of the Study:
- To develop a permanent staining method for Giardia lamblia trophozoites.
- To improve the diagnostic accuracy and ease of Giardia detection.
Main Methods:
- Stool sample smears were prepared and fixed with methanol.
- Staining was performed using a 1% methylene blue solution.
Main Results:
- Methylene blue staining successfully identified Giardia lamblia trophozoites in all 15 positive samples.
- The method did not stain Giardia lamblia cysts.
- 20 stool samples served as negative controls.
Conclusions:
- Methylene blue staining is a simple and effective permanent staining method for Giardia trophozoites.
- This technique allows for the creation of permanent records for diagnostic purposes.
