[Experimental study of suspicious sensitization component screening on Huachansu injection]
Ying Xiao1, Yu-Bin Zhao, Yan-Ming Xie
1Shijiazhuang College, Shijiazhuang 050035, China. yingxiao629@163.com
Objective:
To explore a new experimental method for screening of allergens in post-market traditional Chinese medicine injections by confirming allergens in Huachansu injection.
Method:
First of all, the serum of patients allergic to Huachansu injection were collected, at the same time, the dubious allergen was conjugated to bovine serum albumin (BSA) by EDC coupling procedure to form complete antigen (BNP-BSA), which makes it possible to reproduce the allergic reaction of Huachansu injection in vitro. The histamine liberation ratio, the level of TNF-alpha and Histamine released from RBL-2H3 mast cell were detected; the above data were compared with those obtained in vivo.
Result:
The difference of the histamine liberation ratio, the levels of TNF-alpha and histamine of the resibufogenin-BSA group, group of patients allergic to Huachansu injection were not significant compared with those of normal control group. However, there were significant difference in those data among the cinobufagin-BSA group, the blank control and normal control group (P<0.05).
Conclusion:
The allergen in the serum collected from patients allergic to Huachansu injection is resibufogenin.
