Related Experiment Video
Updated: May 15, 2026
Comprehensive Characterization of Tissue Mineralization in an Ex Vivo Model
Published on: September 27, 2024
Gene expression profiling and histomorphometric analyses of the early bone healing response around nanotextured
Rima M Wazen1, Shingo Kuroda, Clarice Nishio
1Laboratory for the Study of Calcified Tissues & Biomaterials, Department of Stomatology, Université de Montréal, PO Box 6128, Station Centre-Ville, Montreal, QC, H3C 3J7, Canada.
Unlabelled:
While in vitro studies have shown that nanoscale surface modifications influence cell fate and activity, there is little information on how they modulate healing at the bone-implant interface.
Aim:
This study aims to investigate the effect of nanotopography at early time intervals when critical events for implant integration occur.
Materials & Methods:
Untreated and sulfuric acid/hydrogen peroxide-treated machined-surface titanium alloy implants were placed in rat tibiae. Samples were processed for DNA microarray analysis and histomorphometry.
Results:
At both 3 and 5 days, the gene expression profile of the healing tissue around nanotextured implants differed from that around machined-surface implants or control empty holes, and were accompanied by an increase in bone-implant contact on day 5. While some standard pathways such as the immune response predominated, a number of unclassified genes were also implicated.
Conclusion:
Nanotexture elicits an initial gene response that is more complex than suspected so far and favors healing at the bone-implant interface.
