Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 15, 2026

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds
08:02

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

Published on: January 7, 2019

Strategies for developing decellularized corneal scaffolds.

Amy P Lynch1, Mark Ahearne

  • 1Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland.

Experimental Eye Research
|January 5, 2013
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ZEB1 Modifies VE-Cadherin Signaling in Lymphatic Endothelial Cells.

Microcirculation (New York, N.Y. : 1994)·2026
Same author

Influence of fluid shear stress on human limbal epithelial cells.

Biochemistry and biophysics reports·2026
Same author

The splicing factor kinase SRPK1 is a therapeutic target for peripheral vascular disease.

American journal of physiology. Heart and circulatory physiology·2025
Same author

Pharmacological blocking of microfibrillar-associated protein 4 reduces retinal neoangiogenesis and vascular leakage.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Optimization and evaluation of oxygen-plasma-modified, aligned, poly (Є-caprolactone) and silk fibroin nanofibrous scaffold for corneal stromal regeneration.

Biomaterials and biosystems·2023
Same author

Silk fibroin based interpenetrating network hydrogel for corneal stromal regeneration.

International journal of biological macromolecules·2022
Same journal

Tanshinone IIA Inhibits Choroidal Neovascularization and Restores Outer Blood-Retinal Barrier Function in Vldlr Knockout Mice.

Experimental eye research·2026
Same journal

Understanding the Ocular Accumulation of Mefuparib and its N-dealkylation metabolite: Pharmacokinetics, Melanin Affinity, and Cellular Disposition.

Experimental eye research·2026
Same journal

Mitochondrial Dysfunction and Diabetic Retinopathy: Research Progress from Pathogenic Mechanisms to Therapeutic Targets.

Experimental eye research·2026
Same journal

Middle-wavelength green ambient light attenuates lens-induced myopia progression and is associated with suppression of the Wnt/β-catenin signaling pathway in guinea pigs.

Experimental eye research·2026
Same journal

Experimental Corneal Alkali Burn Models: Methodological Standards, Biological Outcomes, and Translational Gaps.

Experimental eye research·2026
Same journal

Subretinal injection in the USH1CR31* pig model leads to chorioretinal atrophy that limits evaluation of efficacy of an AAV-mediated gene therapy.

Experimental eye research·2026
See all related articles

Decellularized corneas serve as promising scaffolds for tissue engineering, overcoming challenges in replicating native corneal structure. Studies evaluate methods to create these scaffolds for functional corneal grafts and vision restoration.

Area of Science:

  • Biomaterials Science
  • Ophthalmology
  • Tissue Engineering

Background:

  • Replicating native cornea's structure and biochemistry in scaffolds is a major hurdle in corneal tissue engineering.
  • Decellularized corneas are emerging as a viable alternative scaffold source for corneal regeneration.
  • Effective decellularization requires removing cellular components and antigens while preserving the extracellular matrix and architecture.

Purpose of the Study:

  • To review strategies for decellularizing corneas for tissue engineering applications.
  • To examine techniques for assessing decellularization effectiveness and scaffold property changes.
  • To evaluate the suitability of decellularized corneas as scaffolds through in vitro and in vivo studies.

Main Methods:

  • Discussing various cell lysis and material removal strategies for corneal decellularization.

More Related Videos

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
07:35

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

Related Experiment Videos

Last Updated: May 15, 2026

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds
08:02

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

Published on: January 7, 2019

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea
07:35

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

  • Analyzing methods to quantify decellularization and evaluate structural, mechanical, and biological changes.
  • Summarizing in vitro and in vivo experimental findings on decellularized corneal scaffold performance.

    • Main Results:

    • Successful decellularization necessitates preserving the native corneal extracellular matrix and architecture.
    • Various techniques exist to assess the degree of decellularization and its impact on scaffold properties.
    • Decellularized corneas have demonstrated potential as scaffolds in preliminary studies.

    Conclusions:

    • Decellularized corneas offer a promising scaffold for engineering functional corneal tissue grafts.
    • Preserving the native corneal structure during decellularization is critical for successful tissue regeneration.
    • Further in vitro and in vivo research supports the use of decellularized corneas for vision restoration applications.