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Published on: April 26, 2013
Remarkable stabilization of antiparallel DNA triplexes by strong stacking effects of consecutively modified
Kenji Yamada1, Yusaku Hattori, Takeshi Inde
1Department of Life Science, Tokyo Institution of Technology, 4259 Nagatsuta, Yokohama 226-8501, Japan.
Abstract:
The consecutive arrangement of 2'-deoxy-6-thioguanosines (s(6)Gs) and 4-thiothymidines (s(4)Ts) in antiparallel triplex-forming oligonucleotides (TFOs) considerably stabilized the resulting antiparallel triplexes with high base recognition ability by the strong stacking effects of thiocarbonyl groups. This result was remarkable because chemical modifications of the sugar moieties and nucleobases of antiparallel TFOs generally destabilize triplex structures. Moreover, in comparison with unmodified TFOs, it was found that TFOs containing s(6)Gs and s(4)Ts could selectively bind to the complementary DNA duplex but not to mismatched DNA duplexes or single-stranded RNA.
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