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Related Experiment Video

Updated: May 15, 2026

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

sCD44 internalization in human trabecular meshwork cells.

Michael J Nolan¹, Tomoyo Koga, Loyal Walker

¹Department of Ophthalmology and Visual Sciences, University of Illinois-Chicago, Chicago, Illinois, USA.

Investigative Ophthalmology & Visual Science

|January 5, 2013

Summary

This summary is machine-generated.

Soluble CD44 (sCD44) is taken up by trabecular meshwork cells and partially moves to mitochondria. This mitochondrial trafficking of sCD44 may contribute to primary open-angle glaucoma (POAG) pathogenesis.

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Area of Science:

Ophthalmology
Cell Biology
Biochemistry

Background:

Soluble CD44 (sCD44) is a potential biomarker for primary open-angle glaucoma (POAG).
Understanding the cellular fate of sCD44 is crucial for elucidating its role in POAG.

Purpose of the Study:

To investigate the internalization of sCD44 by human trabecular meshwork (TM) cells.
To determine if internalized sCD44 is trafficked to mitochondria within TM cells.

Main Methods:

In vitro study using cultured human TM cells.
Incubation with biotinylated sCD44 (b-sCD44) and hypophosphorylated b-sCD44 (-p b-sCD44).
Confocal microscopy was used to track the localization of b-sCD44 and its co-localization with mitochondria.

Main Results:

TM cells internalized b-sCD44 and -p b-sCD44.
Internalization was inhibited by unlabeled sCD44, hyaluronic acid (HA), and an HA-binding peptide.
Significant co-localization of internalized sCD44 with mitochondria was observed (17.4% for -p b-sCD44, 11.7% for b-sCD44).

Conclusions:

sCD44 is internalized by TM cells.
A portion of internalized sCD44 is trafficked to mitochondria.
Mitochondrial trafficking of sCD44 may play a role in POAG pathology.