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Related Concept Videos

Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

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Related Experiment Video

Updated: May 15, 2026

Cryosectioning Yeast Communities for Examining Fluorescence Patterns
07:03

Cryosectioning Yeast Communities for Examining Fluorescence Patterns

Published on: December 26, 2012

Cryosectioning yeast communities for examining fluorescence patterns.

Babak Momeni1, Wenying Shou

  • 1Division of Basic Sciences, Fred Hutchinson Cancer Research Center.

Journal of Visualized Experiments : Jove
|January 5, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a novel cryosectioning method for visualizing the internal spatial organization of microbial communities, specifically yeast. This technique overcomes limitations of optical microscopy for studying complex cell arrangements and gene expression.

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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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Last Updated: May 15, 2026

Cryosectioning Yeast Communities for Examining Fluorescence Patterns
07:03

Cryosectioning Yeast Communities for Examining Fluorescence Patterns

Published on: December 26, 2012

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
07:25

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: January 5, 2021

Area of Science:

  • Microbiology
  • Microbial Ecology
  • Cell Biology

Background:

  • Microbial communities exhibit spatial organization impacting survival and function.
  • Optical sectioning microscopy is limited in depth for dense microbial colonies like yeast due to light scattering.
  • Understanding internal community structure is crucial for microbial ecology.

Purpose of the Study:

  • To develop and present a novel method for visualizing the spatial distribution of cells within microbial communities.
  • To overcome the depth limitations of optical sectioning techniques in dense colonies.
  • To enable detailed analysis of cellular organization and gene expression in yeast communities.

Main Methods:

  • A fixation and cryosectioning technique using methanol and OCT compound was developed.
  • Yeast communities (Saccharomyces cerevisiae) were fixed, infiltrated, frozen, and cryosectioned.
  • Fluorescence imaging was performed on the cryosections to visualize cell distribution.

Main Results:

  • The cryosectioning method successfully revealed the internal spatial distribution of fluorescent cells within yeast colonies.
  • Demonstrated visualization of both cell distribution and gene expression patterns using fluorescent protein markers.
  • The method provides high-resolution imaging of cellular organization in three dimensions.

Conclusions:

  • Fixation and cryosectioning offer a viable alternative to optical sectioning for studying microbial community architecture.
  • This technique allows for deeper penetration and detailed analysis of internal community structures.
  • The method is applicable to Saccharomyces cerevisiae and potentially other microbial communities.