Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The deoxyuridine suppression test in peripheral lymphocytes.

A Remacha1, M J Barceló, M Pastor

  • 1Servicio de Hematología, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.

European Journal of Haematology
|March 1, 1990
PubMed
Summary

The lymphocyte deoxyuridine suppression test (L-dUST) is unreliable for diagnosing megaloblastic changes. Bone marrow deoxyuridine suppression tests (BM-dUST) effectively identified vitamin B-12 and folate deficiencies, unlike the L-dUST.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tissue-Sparing Outcome of Mohs Micrographic Surgery in Squamous Cell Carcinomas.

Actas dermo-sifiliograficas·2020
Same author

In vitro and in vivo antimicrobial activity of sodium colistimethate and amikacin-loaded nanostructured lipid carriers (NLC).

Nanomedicine : nanotechnology, biology, and medicine·2020
Same author

Chronic obstructive pulmonary disease (COPD) in Spain and the different aspects of its social impact: a multidisciplinary opinion document.

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2020
Same author

Preclinical safety of topically administered nanostructured lipid carriers (NLC) for wound healing application: biodistribution and toxicity studies.

International journal of pharmaceutics·2019
Same author

SEOM clinical guideline in nasopharynx cancer (2017).

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2017
Same author

Multidisciplinary management of head and neck cancer: First expert consensus using Delphi methodology from the Spanish Society for Head and Neck Cancer (part 2).

Oral oncology·2017

Area of Science:

  • Hematology
  • Clinical Biochemistry

Background:

  • Megaloblastic anemia is often caused by vitamin B-12 or folate deficiency.
  • Accurate diagnostic methods are crucial for timely treatment.
  • The deoxyuridine suppression test (dU) has been explored for diagnosing these deficiencies.

Purpose of the Study:

  • To evaluate the efficacy of the lymphocyte deoxyuridine suppression test (L-dUST) in diagnosing megaloblastic changes.
  • To compare the L-dUST with the bone marrow deoxyuridine suppression test (BM-dUST).

Main Methods:

  • Investigated the effects of varying deoxyuridine (dU) concentrations on phytohemagglutinin (PHA)-stimulated lymphocytes.
  • Conducted L-dUST and BM-dUST on patients with vitamin B-12 deficiency, folate deficiency, and other conditions, as well as healthy controls.

Related Experiment Videos

Main Results:

  • High dU concentrations were required to counteract spontaneous folate deficiency patterns.
  • BM-dUST successfully differentiated patients with vitamin B-12 or folate deficiency from controls.
  • L-dUST failed to reliably distinguish patients with megaloblastic changes in most cases.

Conclusions:

  • The L-dUST is not a reliable method for diagnosing megaloblastic changes.
  • BM-dUST is a more effective tool for identifying vitamin B-12 and folate deficiencies.