Related Experiment Video
Updated: May 15, 2026

Corneal and Limbal Alkali Injury Induction Using a Punch-Trephine Technique in a Mouse Model
Published on: August 4, 2023
[Accidental staining of corneal nerves by methylene blue]
S Peter1, E Reichart, L Poyntner
1Abteilung für Augenheilkunde, Landeskrankenhaus Feldkirch, Österreich. Silvia.Peter@LKHF.at
Abstract:
A 10-year-old child presented after accidental exposure of the left eye to a blue hair dye containing methylene blue. Mild ocular surface changes and a selective blue staining of the usually invisible corneal nerve fibre bundles were present. Corneal sensitivity was reduced. Despite copious lubrication a transient neurotrophic keratitis developed which did not resolve until corneal sensitivity became normal 2 weeks later. Association of mild chemical burns with neurotrophic keratitis is unusual but is of high clinical relevance as keratitis is a vision-threatening complication.
