Related Experiment Videos

Rapid procedure to determine the DNA base composition from small amounts of gram-positive bacteria

T Ezaki¹, S M Saidi, S L Liu

¹Department of Microbiology, Gifu University School of Medicine, Japan.

FEMS Microbiology Letters

|January 15, 1990

Abstract:

A universal rapid procedure to determine the DNA base composition (mol% guanine + cytosine) of Gram-positive bacteria is described. Cells of Gram-positive bacteria were lysed with achromo-peptidase and the mol% G + C of their DNAs were determined by using high performance liquid chromatography. One ml of a Gram-positive bacterial suspension which matched MacFarland No. 3 standard turbidity was sufficient to determine the mol% G + C within 3 h.

Related Concept Videos

Articles linked to this work by shared authors, journal, and citation graph.

Legionnaires' disease associated with habitual drinking of hot spring water.

Internal medicine (Tokyo, Japan)·2001

[A survival case of severe Legionella longbeachae pneumonia].

Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases·2001

Proposal of Sphingomonas wittichii sp. nov. for strain RW1T, known as a dibenzo-p-dioxin metabolizer.

International journal of systematic and evolutionary microbiology·2001

Proposal of Sphingomonadaceae fam. nov., consisting of Sphingomonas Yabuuchi et al. 1990, Erythrobacter Shiba and Shimidu 1982, Erythromicrobium Yurkov et al. 1994, Porphyrobacter Fuerst et al. 1993, Zymomonas Kluyver and van Niel 1936, and Sandaracinobacter Yurkov et al. 1997, with the type genus Sphingomonas Yabuuchi et al. 1990.

Microbiology and immunology·2000

Arguments against the replacement of type species of the genus Salmonella from Salmonella choleraesuis to 'Salmonella enterica' and the creation of the term 'neotype species', and for conservation of Salmonella choleraesuis.

International journal of systematic and evolutionary microbiology·2000

Burkholderia uboniae sp. nov., L-arabinose-assimilating but different from Burkholderia thailandensis and Burkholderia vietnamiensis.

Microbiology and immunology·2000

See all related articles