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Updated: May 15, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
Radiorespirometric assays for the detection of biogenic sulfides from sulfate-reducing bacteria
J C de Queiroz1, A C de Melo Ferreira, A C A da Costa
1Instituto de Química, Programa de Pós-Graduação em Química, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil.
Aims:
The detection of trace concentrations of biogenic sulfides can be carried out through radiorespirometric assays. The objective of this work was to improve the methodology for detection of H2 S in trace concentrations, to correlate with sulfate-reducing bacterial activity.
Methods And Results:
Serial dilutions of synthetic sea water with a pure culture of Desulfovibrio alaskensis, a mixed anaerobic microbial culture and a natural saline sample from a petroleum offshore platform indicated that dilutions were followed, accordingly, by sulfate reduction.
Conclusions:
Tests performed indicated that increasing the time of incubation of a mixed anaerobic microbial culture contributed to an increase in the sulfate reduction rates, as well as the amount of carbon source and inoculum.
Significance And Impact Of The Study:
The technique here developed proved to be a rapid test for the detection of biogenic sulfides, particularly those associated with corrosion products, being an useful tool for monitoring and controlling oil/water storage tanks, petroleum continental platforms and several types of reservoirs.
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