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A new method for culturing human hair follicle cells on floating mixed collagen membranes
R Kuwana1, S Arase, Y Sadamoto
1Department of Dermatology, Kochi Red Cross Hospital, Japan.
The Journal of Dermatology
|January 1, 1990
Abstract:
A new method for culturing human hair follicle cells without a biological feeder layer has been developed. Dispersed hair follicle cells obtained from plucked hairs have been successfully grown on floating mixed collagen membranes (collagen types I and IV) and have reached a confluent state. They could be subcultured up to twice. Differentiation of the cells was maintained throughout a month in culture. Stratification of the cells and continuous epithelial pavement formation were seen, as in cultured epidermal keratinocytes.