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Updated: May 15, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Increase in effectiveness of holographic particle field reconstruction using superposition procedure
Abhishek Nigam1, P K Panigrahi
1Department of Mechanical Engineering, Indian Institute of Technology, Kanpur, Uttar Pradesh 208016, India.
Abstract:
Noise removal and lesser computational run time of the digital holographic numerical reconstruction procedure are the critical issues for effective and efficient identification of three-dimensional (3D) particle fields. The present study suggests an improved reconstruction procedure based on the superposition principle. The effectiveness of this proposed method is evaluated using both simulated and experimental data of a 3D particle field. Influence of object-particle number density and sample volume depth on the reconstructed particle field is investigated. There is a reduction in computational run time (as high as 50%) and significant increase in reconstruction effectiveness (as high as 7 times increase) due to the proposed method as compared to the literature (Opt. Express 18, 2426, 2010 and Opt. Express 12, 2270, 2004).
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